WebAccording to BioLegend reference, I used 500ng/ml ionomycin and 500ng/ml PMA to stimulate splenocytes as well as GolgiStop (BD product). Medium I used RPMI 1640 with 10% FBS. WebIonomycin is a membrane permeable calcium ionophore produced by the bacterium Streptomyces conglobatus. This ionophore facilitates the transfer of calcium ions (Ca2+) …
Fura-2 AM calcium imaging protocol Abcam
Web7 feb. 2024 · Although IL-2, IL-12, PHA, anti-CD3 plus anti-CD28, and PMA/ionomycin can all activate lymphocytes, PMA/ionomycin is the best one to boost cytokine production with short-term stimulation (4–6 h). This is also in accordance with previous study indicating that PMA/ionomycin can rapidly induce the secretion of IFN-γ by directly activating protein … WebIonomycin also activated (2 microM) and primed (20-200 nM) the PMN oxidase. Dual phosphorylation of p38 MAP kinase and phosphorylation of its substrate activating transcription factor-2 were detected at ionomycin concentrations that prime or activate the PMN oxidase, while dual phosphorylation of p42/44 MAP kinase and phosphorylation of … granitestone family reviews
Lymphocyte proliferation (stimulation index (SI) = % of …
WebProduct Specific Information. Description: This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/p42, or MAPK3/1) when phosphorylated on T202/Y204. ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases ... Web16 okt. 2015 · Ionomycin, free acid (ab120370) HBSS Protocol: Mix Fura-2 AM 50 µg lyophilized by adding 50 µL of DMSO and vortex for 1 min. Store at -20 o C wrapped in foil for light protection. Mix Hank’s Buffered Salt Solution (HBSS-10X) -with final 1x concentration: For 1000 ml, use 850 mLs distilled H 2 0 (18 Mohm), add in order, stirring. Web12 feb. 2024 · Induction of calcium influx e.g. by ionomycin (IO) is the classical pathway leading to PS externalization ( Suzuki et al., 2010) and to ADAM10 activation ( Maretzky et al., 2015 ). Accordingly, IO-treatment induced PS exposure ( Supplementary Figure S1A) and BTC shedding in COS7 cells (Figure 1 A). granite stone emerald green collection